Mice


Mice to genetically manipulate muscle stem cells

Pax7CreERT2: Mice we developed to manipulate fetal, neonatal, and adult muscle stem cells (adult muscle stems are called satellite cells). In the adult, at least 95% of satellite cells are genetically labeled with YFP in Pax7CreERT2/+;R26RYFP/+ mice. These mice are described and used in Murphy et al. 2011, Murphy et al. 2014, and Keefe et al. 2015.  They are available through JAX (use adjacent link). These have been ordered by > 250 labs as of June 2016!


Mice to manipulate muscle connective tissue fibroblasts

Tcf4GFPCre: Mice we developed (in collaboration with Melinda Angus-Hil and Mario Capecchi) to manipulate muscle connective tissue fibroblasts. At P0, Tcf4GFPCre+Neo mice label 58% of the fibroblasts, and no myogeneic cells, while Tcf4GFPCre-Neo mice label 100% of the fibroblasts, but label some myogenic cells (probably myofibers). Both mice are described in Mathew et al. 2011. Contact Gabrielle to obtain mice.

Tcf4CreERT2Mice we developed (in collaboration with Melinda Angus-Hill and Mario Capecchi) to manipulate muscle connective tissue fibroblasts, primarily in the adult. At 6 weeks, Tcf4CreERT2 mice label 45% of the fibroblasts. The mice are described in Murphy et al. 2011Contact Gabrielle to obtain mice.